Types of pipetting equipment and precautions

In fields such as scientific experiments and medical research and development, pipetting equipment is a key tool for accurately measuring and transferring liquids. There are many types and characteristics of each. These equipment are not only related to the accuracy of experimental results, but also directly affect the safety and efficiency of the experimental process. From basic micropipettes to high-precision automated pipetting systems, every pipetting equipment plays an indispensable role.

1. Types of pipetting equipment 1. Features of vented micropipettes (pipetition guns): adjustable, high precision, often used to measure liquids with volumes of 0.1-1000 microliters. 2. Characteristics of positive displacement pipettes: rare, usually used to avoid contamination or handle special substances (such as volatile organic compounds, DNA, etc.). 3. Features of volumetric pipettes (pipettes): fixed volumes (such as 10, 25, 50 ml) and high precision (four significant figures). 4. Features of graduated pipettes (graduated pipettes, graduated pipettes): It has a series of scale lines, can absorb different volumes of liquid, and has a positive and negative error range. 5. Pasteur pipette features: a traditional manual pipetting tool, suitable for transferring large volumes of liquids. 6. Features of multi-channel pipette: It can operate multiple samples at the same time to improve experimental efficiency. 7. Features of the electric pipette gun: It is driven by electricity to reduce manual operations and is suitable for long-term operations or experiments that require high precision.

2. Summary of precautions 1. Choose appropriate pipetting equipment: Choose appropriate pipetting equipment according to experimental needs, including capacity, accuracy, operation method, etc. 2. Calibration: The pipetting equipment needs to be calibrated before use to ensure accurate measurement. 3. Avoid contamination: Use clean pipette tips or straws to avoid cross-contamination. Wash and dry thoroughly after each use. 4. Stable operation: Keep your hands stable when sucking and discharging liquids to avoid liquid splashing or bubbles. 5. Pay attention to safety: Take appropriate safety measures when handling toxic, corrosive or flammable liquids, such as wearing protective glasses, gloves, etc. 6. Maintenance: Perform regular maintenance on pipetting equipment, such as replacing sealing rings, cleaning the interior, etc., to extend service life and maintain accuracy.

3. Common pipetting equipment materials 1. Common plastic materials: polypropylene (PP), polyethylene (PE), polycarbonate (PC), etc. Advantages: Easy to disinfect, most plastic materials can be disinfected through ultraviolet light, alcohol or disinfectant. Easy to use, the plastic material usually has a good feel and is easy to operate. Not easy to break, plastic pipettes are more resistant to falling than glass. Economical and suitable for large-scale laboratory use. 2. Advantages of glass material: acid and alkali resistance, high temperature resistance, corrosion resistance, suitable for pipetting chemicals and other harmful substances. Amazingly resistant to chemical and biological surface substances and stable at high temperatures. Long service life without affecting sample quality. Common materials: borosilicate glass, ordinary glass, etc. 3. Advantages of metal material: The surface is smooth and easy to clean, and it can be automatically steam sterilized. It has good corrosion resistance and is suitable for pipetting various reagents such as organic reagents and chemical reagents. It has good high temperature resistance and is not easy to deform. Common materials: stainless steel, aluminum, etc. 4. Other materials such as PVDF (polyvinylidene fluoride): have excellent chemical corrosion resistance, high temperature resistance and mechanical strength, and are suitable for experimental scenarios with high material requirements.

After discussing the rich variety of pipetting equipment and the precautions that need to be kept in mind when using them, we have a deep understanding of the important role these precision tools play in scientific research and technology development. Each type of pipetting equipment is designed for specific experimental needs. Whether it is a pipette gun that pursues micro-volume precision, a graduated pipette suitable for large-volume transfers, or an automated pipetting system that improves efficiency, they together constitute the experimental Indispensable indoor infrastructure.