The process of screening antibiotics by fully automatic pipetting workstations

With the continuous development of biomedical and drug research, the importance of antibiotic screening in the research and development of new drugs has become increasingly prominent. Traditional antibiotic screening methods usually rely on manual operations, which are not only time-consuming and inefficient, but are also susceptible to interference from human factors. In order to improve screening efficiency and accuracy, a fully automatic pipetting workstation came into being. Through automated operating procedures, it greatly improves the efficiency and accuracy of antibiotic screening.

Full automatic pipetting workstation

1. Functions and advantages of fully automatic pipetting workstationsAs an important automation equipment in modern laboratories, the fully automatic pipetting workstation is mainly used for liquid distribution, sample processing, solution preparation and other work. Its core advantage is that it greatly reduces the error of manual operation and ensures high throughput and high consistency of the experiment. During the antibiotic screening process, researchers need to process a large number of samples and reagents, and the fully automatic pipetting workstation can accurately complete these tasks according to preset procedures, avoiding the tedious steps and possible contamination risks in manual operations.

2. Basic procedures for screening antibiotics by fully automatic pipetting workstations1. Sample preparation and microbial culture (1) Sample collection: Collect microbial samples that may contain antibiotic-producing bacteria from different environments such as soil, water, plants, etc. (2) Microbial culture: Inoculate the collected samples onto an appropriate culture medium and cultivate the microbials. The culture conditions should be adjusted according to different microorganism types, such as temperature, pH, oxygen content, etc., to ensure the normal growth and reproduction of microorganisms. 2. Initial screening and re-sieve (1) Initial screening: Use appropriate methods (such as agar diffusion method, broth dilution method, etc.) to conduct preliminary screening of cultured microorganisms to detect whether they have antibacterial activity. Fully automatic pipetting workstations can improve screening efficiency and accuracy by precisely distributing and mixing reagents, as well as automatic detection and analysis of results. (2) Re-sieve: Further screening of microorganisms with antibacterial activity in the primary screen to determine the stability and specificity of their antibacterial activity. Re-sieving can adopt more precise methods, such as micro broth dilution method, paper diffusion method, etc. Fully automatic pipetting workstations can also provide precise liquid handling and data analysis support at this stage. 3. Extraction and identification of active substances (1) Extraction of active substances: Extract active substances from microorganisms with antibacterial activity in re-sieve. The extraction method should be selected according to the properties of different microbial species and active substances, such as solvent extraction method, ion exchange method, gel filtration method, etc. The fully automatic pipetting workstation can automatically complete the liquid transfer and mixing operations during these extraction processes, improving extraction efficiency and purity. (2) Identification of active substances: Identify the chemical structure and properties of the extracted active substances. The identification method can be spectral analysis, mass spectrometry analysis, nuclear magnetic resonance, etc. Fully automatic pipetting workstations can provide precise sample processing and data analysis support at this stage, helping researchers quickly and accurately determine the chemical structure and properties of active substances. 4. Research on antibacterial mechanisms and animal experiments (1) Research on antibacterial mechanisms: Study the antibacterial mechanism of identified active substances to understand their targets and antibacterial mechanisms. Research methods can be made using molecular biology, cell biology, biochemistry, etc. Fully automatic pipetting workstations can provide precise liquid handling and sample distribution support at this stage, helping researchers better understand the antibacterial mechanisms of active substances. (2) Animal experiments: Animal experiments are conducted on compounds with antibacterial activity to evaluate their safety and effectiveness. Animal experiments can be performed using mice, rats, rabbits and other animals. Fully automatic pipetting workstations can provide precise liquid handling and data analysis support at this stage, helping researchers quickly and accurately evaluate the safety and effectiveness of compounds. 5. Clinical trials and new drug declarations (1) Clinical trials: Clinical trials are conducted on compounds that show good safety and effectiveness in animal experiments to evaluate their safety and effectiveness in humans. Clinical trials require strict approval and regulatory procedures. Although the fully automatic pipetting workstation has relatively limited roles at this stage, its precise liquid handling and data analysis support in previous experiments has laid a solid foundation for the success of clinical trials. (2) New drug declaration: New drug declaration is made for compounds that show good safety and effectiveness in clinical trials, and can only be put on the market after obtaining approval from the drug regulatory authorities. The precise liquid processing and data analysis support provided by the fully automatic pipetting workstation throughout the screening and R&D process provides strong guarantees for the successful declaration of new drugs.

The application of fully automatic pipetting workstations in antibiotic screening has greatly improved the high throughput and high accuracy of the experiment, reduced artificial intervention and operational errors, and provided strong support for drug research and development and biomedical research.

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