Steps to extract RNA samples from fully automatic pipetting workstations

With the continuous development of life science research, RNA extraction technology is becoming increasingly widely used in the fields of gene expression analysis, disease diagnosis, etc. The emergence of fully automatic pipetting workstations has greatly improved the efficiency and accuracy of RNA extraction. Opentrons editors will provide a detailed introduction to how to extract RNA samples using a fully automatic pipetting workstation and ensure efficient and accurate operation.

Full automatic pipetting workstation

1. PreparationBefore starting RNA extraction, the following materials and equipment are first required: 1. Fully automatic pipetting workstation: Make sure that the equipment has passed the quality control and that the pipetting system is normal. 2. RNA extraction kit: common ones such as Trizol reagents. 3. Sample: Usually a cell, tissue or other biological sample. 4. Pipet head and consumables: Ensure the sterility and applicability of the pipet head and consumables. 5. Operating software for presetting the extraction steps: According to the instructions of the extraction kit, set the extraction steps in the operating system of the fully automatic workstation in advance to ensure that the device can execute according to the specified program.

2. Setting the extraction programOne of the key advantages of a fully automatic pipetting workstation is its high degree of automation. Operators need to write appropriate operating procedures according to the requirements of the RNA extraction kit. Generally, the steps include: 1. Sample lysis: Add lysis buffer and mix. 2. Centrifugation and stratification: Separate the cell lysate from RNA by centrifugation. 3. Sample cleaning: remove impurities and DNA. 4. RNA precipitation: Finally, RNA is precipitated by centrifugation. Fully automatic pipetting workstations can perform these steps accurately to reduce errors caused by human operations. When setting, it is necessary to ensure that the time, temperature and operation of each step meet the requirements of the kit.

3. RNA extraction stepsThe specific extraction steps will vary depending on the kit used, but usually include the following basic procedures: 1. Sample lysis: Put the sample (such as cells, tissues, etc.) of the RNA to be extracted into the reaction tube of the workstation. The fully automatic pipetting workstation will automatically add lysis buffer according to the set procedure and mix evenly through the pipetting device to ensure that the cells or tissues are completely lysed and RNA is released. 2. Centrifugation: The lysed sample needs to be centrifuged to separate RNA from other cellular components (such as DNA, proteins). The fully automatic pipetting workstation automatically performs this process to ensure accurate centrifugation speed and time to improve RNA purity. 3. Cleaning steps: RNA needs to remove impurities such as DNA and protein through a series of cleaning steps. The fully automatic pipetting workstation will automatically add cleaning buffer and perform pipetting, mixing and centrifugation operations. This process is very important because the presence of impurities may interfere with subsequent analysis. 4. RNA precipitation: The washed RNA needs to be extracted through the precipitation step. The fully automatic pipetting workstation will accurately control the added precipitation solution and centrifugation time to make RNA precipitation purer. 5. RNA dissolution: Finally, the extracted RNA is dissolved in appropriate buffer for preparation for quantitative and subsequent analysis.

4. RNA quality controlAfter the extraction is completed, the quality of RNA is crucial and directly affects the results of subsequent experiments. Common quality detection methods include: 1. Spectral absorbance method (NanoDrop): measure the concentration and purity of RNA, and usually determine whether there is protein contamination by the ratio of 260/280. 2. Agarose gel electrophoresis: detect the integrity of RNA and ensure no degradation. 3. Agilent Bioanalyzer: Use special equipment to perform RNA quality testing to provide more accurate quality assessment. The precise operation and automated procedures of fully automatic pipetting workstations can effectively improve the consistency and reliability of RNA extraction, reduce differences between samples, and ensure the stability of extraction results.

The fully automatic pipetting workstation provides an efficient and accurate solution for RNA extraction. Through precise pipetting and centrifugation operations, the automated step setting not only improves the speed of RNA extraction, but also reduces the errors that may be caused by human operations. Properly setting up the operating procedures and choosing the appropriate kit can ensure that the extracted RNA is of high quality and good purity, and is suitable for various subsequent experimental analyses.

Related reading recommendations

6 resources for life scientists working from home

Use Opentrons for COVID-19 testing

Instruction Manual for Fully Automatic Pipe Workstation

How to calibrate OT-2 polypeptide desalination workstation

The embodiment of fully automatic pipetting workstations in robotics technology

Application of fully automatic pipetting workstations in cell culture