What are the storage conditions for protein purification BUFFER

BUFFER (buffer) plays a crucial role in protein purification. It not only maintains the stability of the protein, but also ensures that factors such as pH value and ionic strength are maintained within the appropriate range during the experiment. However, the storage conditions of BUFFER directly affect its effect, so it is crucial to understand the correct storage method of BUFFER.

Protein purification BUFFER

1. Storage temperature1. Short-term storage (such as several days to several weeks): Most protein purified BUFFER can be stored in a 4℃ refrigerator. Low temperatures can slow down the degradation and degeneration of ingredients in BUFFER, ensuring its effect and function. 2. Long-term storage (such as months to years): For BUFFERs that require long-term storage, they should be stored at a temperature of -20℃ or lower. However, it should be noted that some special chemical reagents (such as DTT and other reducing agents) may be unstable in low temperature environments, so the storage temperature should be adjusted according to the specific ingredients.

2. Storage container1. Container material: Clean, pollution-free glass or high-quality plastic bottles should be selected as storage containers. Glass bottles can avoid chemical reactions or trace impurities contamination caused by plastic bottles. 2. Sealing: The storage container should have good sealing properties to prevent moisture or impurities from entering the BUFFER, resulting in changes in ingredients and pollution.

3. Prevent recurrence of freeze-thaw1. Freeze-thaw is an important factor affecting the stability of BUFFER. Frequent freeze-thawing processes may cause changes in the composition in BUFFER, especially in pH or solubility. 2. It is recommended to divide the BUFFER into small vials or tubules, and only the required part is taken each time to avoid repeated freeze-thawing of the entire storage bottle.

4. Validity period and quality control1. The validity period of BUFFER depends on its composition and storage conditions. BUFFER containing protease inhibitors, antioxidants or other active ingredients should be used in the short term as much as possible. 2. BUFFER stored for a long time should undergo quality inspection before use, such as detecting pH value, color changes, etc., to confirm whether it still has the original functions.

5. Preservatives and protective agents1. For BUFFERs that require long-term storage, appropriate preservatives and protective agents (such as glycerin, sucrose, etc.) can be added to extend its storage period and prevent the growth of bacteria or fungi. 2. However, it should be noted that not all BUFFERs are suitable for adding preservatives. Therefore, you should carefully read the relevant literature or product instructions before adding to ensure that they have no negative impact on the protein and experimental process.

6. Light control1. Ultraviolet irradiation may lead to the degradation of certain components in BUFFER. Therefore, direct exposure of BUFFER to strong light should be avoided. 2. It is best to choose a cool and light-proof environment when storing to reduce the impact of ultraviolet rays on BUFFER components.

The storage conditions of protein purification BUFFER are directly related to its use effect. Correct storage methods can extend the validity period of BUFFER and ensure the stability of experimental results. When storing BUFFER, you must follow basic principles such as temperature, container and light protection, and check its status regularly to avoid affecting the accuracy of the experiment due to improper storage.

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