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PCR instrument, also called polymerase chain reaction instrument, is an indispensable equipment in molecular biology research and is mainly used to amplify DNA.
1. Main types
According to different needs, PCR instruments are divided into the following types:
1. Ordinary PCR instrument: suitable for most DNA amplification experiments, meeting the needs of scientific research and clinical testing.
2. Gradient PCR instrument: It can set multiple temperature gradients in the same experiment to optimize PCR reaction conditions, especially suitable for amplification of unknown DNA annealing temperature.
3. Real-time fluorescence quantitative PCR instrument: By detecting changes in fluorescence signals during the PCR amplification process, quantitative analysis of DNA or RNA can be achieved. It is widely used in medical testing, pathogenic microorganism detection and gene expression research.
4. Digital PCR machine: Divide the sample into thousands of tiny reaction chambers, independently amplify and detect the DNA molecules in each reaction chamber, and achieve absolute quantification of the target DNA molecules.
2. Instrument characteristics
1. High sensitivity: can detect extremely small amounts of DNA template.
2. High specificity: through the specific binding of primers, the accuracy of the amplified product is ensured.
3. Rapidity: Complete DNA amplification in a short time.
4. Diversity: Different types of PCR instruments are suitable for different experimental needs, such as genotype analysis, gene mutation detection, pathogen identification, etc.
3. Application fields
PCR instruments play an important role in multiple fields:
1. Molecular biology research: used for gene cloning, gene expression analysis and gene mutation detection.
2. Medical testing: used for detection of pathogenic microorganisms, diagnosis of genetic diseases and detection of tumor markers.
3. Biotechnology research and development: widely used in fields such as genetic engineering, protein engineering and drug research and development.
4. Suggestions on purchasing and maintenance
Purchasing suggestions:
1. Choose the appropriate PCR instrument type according to experimental needs.
2. Consider brand, performance and price, and choose products with high cost performance.
3. Pay attention to the after-sales service and maintenance guarantee of the instrument.
Maintenance recommendations:
1. Clean the inside and outside of the instrument regularly to keep it clean and hygienic.
2. Operate correctly according to the instructions to avoid misoperation and damage.
3. Calibrate instruments regularly to ensure the accuracy and reliability of experimental results.
4. Handle instrument failures in a timely manner to avoid affecting the experimental progress and results.
PCR instruments play an important role in molecular biology research and medical testing. As technology continues to advance, the application fields of PCR instruments are also expanding. If you are interested in PCR-related automation solutions and instruments, you can contact our customer service or call the hotline for details. Our product prices are transparent and inquiries are welcome.
The experienced service team and strong production support team provide customers with worry-free order services.
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