What does next-generation sequencing of genes mean?

Next-generation gene sequencing refers to the subsequent mutation status detection and mutation measurement of multiple mutation points of multiple genes at the same time to guide subsequent targeted treatments. Gene second-generation sequencing, also known as next-generation sequencing, can simultaneously evaluate the status of hundreds of thousands to hundreds of thousands of genes. With the continuous development of technology, it provides great potential for subsequent targeted detection. Convenience makes up for many of the previous shortcomings.

For example, when performing small biopsy specimens, there is not enough tissue for subsequent multiplex PCR testing, nor is there enough tissue for repeated verification of multiple indicators and targets. Next-generation sequencing perfectly solves this problem. Second-generation sequencing technology has greatly reduced sequencing costs, while also greatly increasing sequencing speed and maintaining high accuracy. It used to take 3 years to complete the sequencing of a human genome, but using second-generation sequencing technology only takes 1 week. , but the sequence read length is much shorter than that of first-generation sequencing technology. The second-generation gene sequencing process includes steps such as sample preparation, DNA or RNA extraction, library construction, PCR sequencing, and data analysis. Different reagents and operating methods need to be used in each step to ensure the accuracy and reliability of sequencing results.